RESUMO
Alterations in the erm(A) regulatory region of six clinical isolates of Staphylococcus epidermidis and one of Staphylococcus haemolyticus displaying a constitutive resistance phenotype were investigated. Anovel deletion of 10 bp with respect to the corresponding sequence of Tn554 was identified in the attenuator of a constitutively expressed erm(A) gene of one of the S. epidermidis isolates. Thus far, this is the smallest deletion conferring constitutive resistance in the translational attenuator of erm(A) in a naturally occurring S. epidermidis strain of human origin.
Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Macrolídeos/farmacologia , Metiltransferases/genética , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/genética , Sequência de Bases , Farmacorresistência Bacteriana/genética , Regulação Bacteriana da Expressão Gênica , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Biossíntese de Proteínas/genética , Sequências Reguladoras de Ácido Nucleico/genética , Deleção de Sequência , Staphylococcus epidermidis/isolamento & purificaçãoRESUMO
Alterations in the erm(A) regulatory region of six clinical isolates of Staphylococcus epidermidis and one of Staphylococcus haemolyticus displaying a constitutive resistance phenotype were investigated. A novel deletion of 10 bp with respect to the corresponding sequence of Tn554 was identified in the attenuator of a constitutively expressed erm(A) gene of one of the S. epidermidis isolates. Thus far, this is the smallest deletion conferring constitutive resistance in the translational attenuator of erm(A) in a naturally occurring S. epidermidis strain of human origin (AU)
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Assuntos
Staphylococcus epidermidis/genética , Antibacterianos/farmacologia , Metiltransferases/genética , Macrolídeos/farmacologia , Proteínas de Bactérias/genética , Staphylococcus epidermidis/isolamento & purificação , Staphylococcus epidermidis/patogenicidade , Deleção Cromossômica , Elementos de DNA Transponíveis/genética , Sequência de BasesRESUMO
High-level aminoglycoside resistance was assessed in 190 commensal erythromycin-resistant alpha-hemolytic streptococcal strains. Of these, seven were also aminoglycoside-resistant: one Streptococcus mitis strain was resistant to high levels of kanamycin and carried the aph(3 ')-III gene, four S. mitis strains were resistant to high levels of streptomycin and lacked aminoglycoside-modifying enzymes, and two S. oralis strains that were resistant to high levels of kanamycin and streptomycin harbored both the aph(3 ')-III and the ant(6) genes. The two S. oralis strains also carried the ant(6)-sat4- aph(3 ' ')-III aminoglycoside-streptothricin resistance gene cluster, but it was not contained in a Tn5405-like structure. The presence of this resistance gene cluster in commensal streptococci suggests an exchange of resistance genes between these bacteria and enterococci or staphylococci.
Assuntos
Aminoglicosídeos/farmacologia , Estreptococos Viridans/efeitos dos fármacos , Estreptococos Viridans/genética , Sequência de Bases , DNA Bacteriano/química , DNA Bacteriano/genética , Farmacorresistência Bacteriana Múltipla/genética , Endocardite Bacteriana/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Família Multigênica , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Infecções Estreptocócicas/microbiologia , Estreptococos Viridans/metabolismoRESUMO
High-level aminoglycoside resistance was assessed in 190 commensal erythromycin-resistant alpha-hemolytic streptococcal strains. Of these, seven were also aminoglycoside-resistant: one Streptococcus mitis strain was resistant to high levels of kanamycin and carried the aph(3)-III gene, four S. mitis strains were resistant to high levels of streptomycin and lacked aminoglycoside-modifying enzymes, and two S. oralis strains that were resistant to high levels of kanamycin and streptomycin harbored both the aph(3)-III and the ant(6) genes. The two S. oralis strains also carried the ant(6)-sat4- aph(3)-III aminoglycoside-streptothricin resistance gene cluster, but it was not contained in a Tn5405-like structure. The presence of this resistance gene cluster in commensal streptococci suggests an exchange of resistance genes between these bacteria and enterococci or staphylococci (AU)
No disponible
